micro-aspiration system of autologous bone marrow tissue for stem cell therapy
The most studied source of mesenchymal stem cells (MSCs) is the bone marrow. They are particularly abundant in the fraction of mononucleated cells.
MSCs are conductors of tissue reparation and regeneration, function that are expleted through the secretion of trofic mediators. Therefore, MSCs are likely the most interesting cells when it come to orthopedic applications given their potential to differentiate both in bone and cartilage.
A comparative study on the quantity of nucleous cells generated from different bone sources highlighted how the bone marrow aspirate from the posterior iliac crest provides the highest concentration of nucleous cells, (25.1-54.7)x10 cells/ml. In decreasing order of cell abundance have been ranked has best autologous source of MSCs respectively: inferior iliac crest, proximal humerus, distal femur, vertebral bodies, humerus head, proximal tibia, heel bone, and distal tibia.
The quantity of MSCs in the bone marrow aspiration depends, as well as on factors like the age of the patient, on the tecnique of aspiration employed, due to the risk of diluition of peripheral blood.
IT HAS BEEN PROVED THAT BY PERFORMING MICRO ASPIRATIONS WITH SMALL VOLUME SYRINGES, HIGHER CONCENTRATIONS OF MONONUCLEAR CELLS BM-MNCS CAN BE OBTAINED.
CellColt creates an exclusively later and radial aspiration flow, thanks to the presence of an internal aspiration closed-end cannula. This characteristic minimize the dilution of the marrow aspirate with peripheral blood, an improvement compared to the traditional opend-end cannulas which collect an aspirate with high presence of peripheral blood.
LATERAL MULTI RADIAL ASPIRATION FLOW ON 4 LEVELS
MAXIMIZES RETRIEVAL OF PROGENITOR AND MESENCHIMAL CELLS
MINIMIZES DILUITION WITH PERIPHERAL BLOOD
MICRO ASPIRATION OF 2ML
The patented "Sliding Block" , located on the trocar handle, allows to retreat the internal aspiration cannula alligning and opening new side holes on four different levels.
The alternated opening of 6 out of 12 of holes located along the trocar, allows the aspiration of the marrow on different levels of depth. The trocar exploit a larger area of aspiration inside the marrow, using only one site of entry.
The execution of the micro aspirations of 2ml on multiple levels maximizes the capacity of cell retrieval.